Christian Peeters and Robin Crewe.
Male biology in the queenless ponerine ant, Ophthalmopone berthoudi (Hymenoptera: Formicidae).
Psyche 93(3-4):277-284, 1986.

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MALE BIOLOGY IN THE QUEENLESS PONERINE ANT OPHTHALMOPONE BERTHOUDI
(HYMENOPTERA: FORMICIDAE)
BY CHRISTIAN PEETERS* AND ROBIN CREWE
Department of Zoology, University of the Witwatersrand, Johannesburg 2001, South Africa
Various ponerine ants exhibit significant modifications in their pattern of male dispersal, and this is associated with changes in the queenright social structure. In some species the queen caste has become permanently wingless (= ergatoid queens), and in others it has been replaced by mated laying workers (= gamergates; Peeters and Crewe, 1984). Thus, male nuptial flights take on new character- istics since they have to locate flightless sexual partners. Data on male behavior are only available for a few of the ponerine species without a queen caste, but generally males disperse individually and orientate to foreign nests, around which mating then occurs. Brown (1953) observed low-flying males entering nests in two species of Rhytidoponera. Mating can occur outside the nest entrances (e.g. in R. chaiybaea; Ward, 1981), or inside the nest (e.g. in Diacamma rugosum; Wheeler and Chapman, 1922).
Ophthalmopone berthoudi Fore1 is permanently queenless, and details of its reproductive system and polydomous organization appear elsewhere (Peeters and Crewe, 1985, MS). This paper deals with the pattern of male behavior in the field and the characteristics of male production in a breeding system made up exclusively of laying workers.
Colonies of Ophthalmopone berthoudi were studied in one local- ity in Mkuzi Game Reserve (north-eastern Natal, South Africa), during 1981-1983. Observations were made throughout the year, *Present address: School of Zoology, University of New South Wales, P.O. Box I, Kensington N.S.W., Australia 2033.
Manuscript received by the editor May 20, 1986.



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278 Psyche [vo~. 93
but the ones specifically reported in this paper were made during the period of male activity (January-April). Male behavior was usually studied near colonies under intensive study (Peeters, 1984). In such colonies the location of all the nest entrances was known (colonies are polydomous), and all the workers active outside the nests had been color-marked with individual codes. In addition, a few males were marked on the thorax and then released. The presence of males was determined by observation of their activity outside nests and by examination of the contents of excavated nests. Dates of male activity
Normal winged males are produced in this species and were found inside most nests excavated during January-April (Table 1). This limited period of male production was confirmed by finding male pupae during January-April only. A subjective impression is that the number of males present above ground reached a peak in Feb- ruary. Excavations also revealed that males are present in every nest of a colony. However, nests collected in the same month could contain different numbers of males (Table 1). During January and February, a few males were seen to be carried between the nests of a colony. This carrying did not follow any organized pattern, and occurred together with the recruitment of workers and brood. Many of the cocoons that were transferred between nests during that period contained male pupae (A sample of cocoons then found in the nests yielded 70 male pupae and 248 worker pupae). The investment in male production does not appear exceptional in this queenless species; a colony (464 workers) with five nests excavated in February 1982 yielded 60 males (Table l), and this is in addition to those that had already departed as well as pupae. Dispersal behavior
Every day during a three-week observation period in January- February 1982, a few males (1-8) left from each of six nests under intensive observation. Departing males left the nests, often climbed up low vegetation and flew off. Once on the wing, they could no longer be followed. The time of departure (9HOO to 12HOO) often coincided with the period when workers were no longer active on the surface because of high soil temperatures. Male exit times appeared not to be affected by cloudy or cooler weather.



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19861 Peelers & Crewe-Male biology in Ophthalmopone 279 Table 1. Size of the male population in nests excavated during January-April. Males were not present at other times of the year. Date of
Number of nests Number of males found in each nest excavation excavated (together with number of adult workers) January 1981 4 # 5(145), 0(84), 6(142), lO(227) January 1982 1 170 140)
January 1983 3 # 3(116), 9(222), 6(121)
February 1982 5 + 7(20), 9(80), 19(168), 13(72), 12(124) March 1982 2 + 0(119), O(77)
April 1981 3 + O(3 1 8), 0(75), 1 (445)
April 1983 2 + 0(106), 2(121)
# from 2 colonies'
+ from same colony
Evidence that males remain in their natal nests until they are physiologically ready to mate was adduced from the following observations. An adult male that was painted while being carried between two nests, remained inside the second nest for nine days before it left and flew off. Dissection of males collected during excavations revealed that there was little or no sperm in the vasa deferentia and ejaculatory ducts of many of them. After the initial dispersal flight, males alighted on the ground and appeared to search for nests haphazardly. They walked quickly with frequent changes of direction, and investigated little holes and depressions in the soil. They regularly climbed up short grass stems or low vegetation from which they flew off, often for only a short distance. This behavior was interspersed with ground searches. On a number of occasions, males were observed either landing very close to nest entrances, or walking straight towards occupied nests shortly after landing. Five marked males were observed outside one nest on two successive days, indicating that after locating a foreign nest, Behavior around the entrances of foreign nests During the period of their activity, males were observed waiting immobile outside nest entrances, either on the ground or on top of short grass stems. The working assumption was that such males did not originate from these nests, because they always flew away from their natal nests. Some nests frequently had many males in their vicinity, while other nearby nests seldom had any around them. Males usually investigated entrance holes with their antennae and hesitantly walked in; some ran out immediately afterwards. Individ- ual males were repeatedly evicted from nests by workers (in nests



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280 Psyche [vo~. 93
under intensive study, these were often marked workers, i.e. active on the surface). Males were held by their legs, wings or antennae, and resisted fiercely; some managed to struggle free. After releasing the males in the vicinity of the nests (30 cm -1 m away), the workers ran back into the entrance holes. The uninjured males cleaned their antennae and then immediately attempted to enter the nests again. On some occasions a number of workers cooperated in the eviction of foreign males, and some workers also chased males when they came across them outside the nests. Eviction did not always follow a male's entrance, and some marked males remained underground for at least 15 minutes.
In Ophthalrnopone berthoudi copulation was never observed above ground, and it is inferred that it occurs exclusively inside foreign conspecific nests. This is an unusual situation in ants, who usually mate some distance from the nests. However, copulation can take place in the immediate vicinity of nests in queenright and queenless ponerines, and in socially parasitic myrmicines (e.g. Har- pagoxenus; Buschinger and Alloway, 1979). In Rhytidoponera chalybaea, in which colonies have either a queen or gamergates, large numbers of workers and males mill around nest entrances, and males make repeated attempts to mate with workers (Ward, 1981). However, males also enter nests and may mate with workers there. In the queenless R. metallica, workers attract males by the release of a pygidial gland pheromone; this distinct behavior ('sex- ual calling') occurs outside the nest entrances (Holldobler and Has- kins, 1977). The pygidial gland has been found in 0. berthoudi (Villet et al., 1984), and we speculate that if young workers release this sex pheromone, they only do so inside the nests and hence encounter males underground. Sexual calling was never observed inl the field or in the laboratory.
Direct data are not available on the activities of males inside foreign nests, and the occurrence of mating is inferred from the large proportion of inseminated workers in nests excavated after the period of male activity (Peeters and Crewe, 1985). The existence of many gamergates in some nests (up to 108) strongly suggests that males copulate more than once; otherwise, such nests would need to be visited by larger numbers of males than we observed entering any



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1 9861
Peelers & Crewe- Male biology in Ophthalmopone 28 1
nest. The substantial variations in the percentages of gamergates present in different nests at any one time of the year (Peeters a n d Crewe, 1985) suggest that the number of male visits to a particular nest is irregular. Some nests may be located more often than others, and consequently varying numbers of young workers become mated. In polydomous colonies such as these, gamergates can be transferred between nests and, hence, a colony should survive from year to year as long as one of its nests is visited by males. The exit of males from their natal nests is not coordinated, and they disperse over a period of a few weeks. This is different to the situation in queenright species where the emergence of all the male and female reproductives is synchronized in time (e.g. in Campcfno- tus herculeanus, through the release of a mandibular gland pluero- mone by the males; see HQlldobler and Bartz, 1985). Dispersal is then often associated with the initiation of new nests, which must occur during optimal environmental conditions (e.g. after rain j - In contrast, copulation in 0. berihoudi is not followed by independent colony foundation by the mated workers, because colonies repro- duce by fission (Peeters, 1984). Thus it is no longer selectively advantageous for males to disperse simultaneously in response to a specific environmental cue. However, males continue to be pro- duced only during a short period of the year. Unmated workers show no ovarian activity in 0. berthoudi, and haploid eggs are laid exclusively by gamergates (Peeters and Crewe, 1985). Egg fertiliza- tion is thus a voluntary act by the mated workers, and males are produced following the first summer rains. Sperm exhaustion is unlikely since individual gamergates lay relatively few eggs d ix ring their lifetime. It is not known whether all the gamergates in a nest produce haploid eggs; the inter-nest transfer of male adults and pupae would make this hard to determine. The importance of chemical attractants during nest location remains unclear. In Leptogenys ocellifera, a ponerine with ergasaid queens, dispersing males search for the chemical trails that lead from the nests into the surroundings (Maschwitz and Miihlenberg, 19751, and mates of Megaponera foetens follow trails laid by workers during raids on termite nests (Longhurst and Howse, 1979). This is impossible in 0. berthoudi because continuous trails are: not laid. There is evidence that discrete scent marks are deposited on the substrate by inexperienced foragers (Peeters and Crewe, MS>- but this may be of no use to males. It is conceivable that the pygZdia1



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282 Psyche [vo~. 93
gland secretions also work as a long-distance attractant. In addition to signalling sexual receptiveness to the males inside the nests, these volatile secretions (which are produced by many workers) may dif- fuse out of the nests and be perceived by searching males. Males of 0. berthoudi need to enter foreign nests in order to find sexual partners. The colony units have distinct identities (Peeters, 1984), and alien males are recognized as different by workers, which then attempt to remove them from the nest; similar hostility is also displayed in R. chalybaea (Ward, 198 1). This aggression contrasts with the acceptance of alien males by workers in ponerine species with ergatoid queens, e.g. males in Leptogenys and Megaponera were not attacked following their entry into foreign colonies (Wheeler, 1900; Longhurst and Howse, 1979). In the queenless Dinoponera gigantea, Overal(1980) observed a male being carried into a nest by a forager. Carrying of males in 0. berthoudi was always between the nests of a single polydomous colony and is thus not equivalent to the observations made by Overal. Access by males to foreign nests may be facilitated by the fact that the older workers that perform activities on the surface and are responsible for the evictions, are usually not active during the daily peaks of male activity. The younger workers confined inside the nests are those likely to become mated (Peeters and Crewe, 1985), and these probably do not behave aggressively towards foreign males. If the queenright ancestors of this species exhibited the typical formicid pattern of reproduction, then male and female reproduc- tives would have been produced seasonally. With the change to worker reproduction, the sexually-attractive workers do not dis- perse from their nests prior to mating, and mating is no longer coupled with colony foundation, hence the times of male activity no longer need to be synchronized with female activity periods or with appropriate environmental conditions for colony foundation. This relaxation of the selective pressures on the timing of male dispersal has resulted in an extended mating period. Nonetheless, male activ- ity remains seasonal. This has no adaptive significance in 0. ber- thoudi, because young workers that can be mated occur throughout the year. However it has the effect of ensuring that an adequate number of infertile workers are present in the colonies.



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I 9861 Peelers & Crewe- Male biology in Ophthalmopone 283 We are grateful to R. H. Crozier for comments on the manu- script. We thank the Natal Parks Board for permission to work in Mkuzi Reserve, and Peter Goodman for his hospitality. This work was supported by grants from the University of the Witwatersrand and the Council for Scientific and Industrial Research. BROWN, W. L.
1953.
Characters and synonymies among the genera of ants. Part I. Breviora, Mus. Comp. Zool., 11: 1-13.
BUSCHINGER, A. AND T. M. ALLOWAY
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Sexual behaviour in the slave-making ant, Harpagoxenus canadensis M. R. Smith, and sexual pheromone experiments with H. canadensis, H. americanus (Emery), and H. sublaevis (Nylander) (Hymenoptera; For- micidae). Z. Tierpsychol., 49: 1 13- 1 19. H~LLDOBLER, B. AND C. P. HASKINS
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Sexual calling behavior in primitive ants. Science, 195: 793-794. H~LLDOBLER, B. AND S. H. BARTZ
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LONGHURST, C. AND P. HOWSE
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Zur Jagdstrategie einiger orientalischer Leptogenys-Arten (Formicidae: Ponerinae). Oecol., 20, 65-83.
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PEETERS, C. P.
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Social organization, breeding biology and the process of reproductive differentiation in Ophthalmopone berthoudi Forel, a ponerine ant. Unpublished Ph.D. Thesis, University of the Witwatersrand Johannes- burg, South Africa.
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Insemination controls the reproductive division of labour in a ponerine ant. Naturwiss., 71: 50-5 1.
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Worker reproduction in the ponerine ant Ophthalrnopone berthoudi: an alternative form of eusocial organization. Behav. Ecol. Sociobiol., 18: 29-37.




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Psyche
[Vol. 93
MS. Foraging and recruitment in ponerine ants: solitary hunting in the queenless Ophthalmopone berthoudi (Hymenoptera: Formicidae). Submitted.
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